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文献和实验Using Mini-genes to Identify Factors That Modulate Alternative Splicing
Many genetic mutations result in the disruption of (alternative) splicing. Prime examples are the SMN1 and SMN2 genes: a silent mutation in SMN2 leads to the skipping of the constitutive exon 7 in the majority of SMN2 transcripts
itself was adapted from one obtained by Upstate. The immunohistochemistry procedure, adapted from Biomeda's manual, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole
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