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文献和实验Transmigration of Human CD34+ Cells
to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.
Generation of TransgenicT Cells from Human CD34+Cord Blood Cells
, more committed CD34+ progenitors expressing CD38, and lineage markers like CD7 or IL-7R can generate T cells in the thymus (1 ). Cord blood, fetal liver, bone marrow, and peripheral blood CD34+ cells can generate T cells. These progenitors differ
Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
biological properties of HSCs. We describe here methods for short-term ex vivo culture and gene transfer into human HSCs. Cord blood-derived HSC gene transfer can be tuned to limit the average level of vector integration or instead to maximize
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