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文献和实验Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
methodologies for the measurement of hydrogen peroxide use horseradish peroxidase (HRP) to catalyze oxidation of colorimetric or fluorescent compounds. These drawbacks limit their stability and reproducibility. Materials PMT luminometer
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
Total Bacterial RNA Labeling with Random Hexamers.
Total Bacterial RNA Labeling with Random Hexamers. Arkady Khodursky, Ph.D. Email: khodursk@cmgm.stanford.edu Updated: 2/10/99 20-25ug of total RNA in 10-14 ul of diH20 should be purified using RNA kit or hot phenol extraction
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