- ￥4573
- 赛默飞（Thermo Fisher）
- C1ORF115-101AP
- 2025年07月15日
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文献和实验media: Ap 50 μg/ml, Km 50 μg/ml. For BACs and low copy vectors, on plates and in liquid media: chloramphenicol (Cm) 20 μg/ml, Ap 50 μg/ml and Km 15 μg/ml. Tetracycline (Tc) concentrations for selection of pSC101BADγβαA[tet] was 5 and 3 μg/ml on plates
CLONING FROM BEGINNING TO END 详细的克隆操作过程【UCSF】
,sometimes it is necessary to dephosphorylate the ends of the vector so as to prevent religation before the insert can ligate to vector.If this is necessary: VECTOR DEPHOSPHORYLATION (AP/CIP): a.after digesting (it is best to do this before running on the gel and Gene
): 5’-AAGCTTTTTTTTTTTA-3’ ②H-T11C(2uM): 5’-AAGCTTTTTTTTTTTC-3’ ③H-T11G(2uM): 5’-AAGCTTTTTTTTTTTG-3’（2）5’ 随机引物：Kit引物：①H-AP1(2uM): 5’-AAGCTTGATTGCC-3’ ②H-AP2(2uM): 5’-AAGCTTCGACTGT-3’ ③H-AP3(2uM): 5’-AAGCTTTGGTCAG-3’ ④H-AP4(2uM): 5’-AAGCTTCTCAACG-3’ ⑤H-AP5(2uM
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