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文献和实验MATERIALS T-cell: CD2 IgG2b, 01171D B-cell: B220 (CD45R) IgG2a, 01121A CD3 IgG2b, 28001D Monocytic: Mac1 (CD11b) IgG2b, 01711A CD5 IgG2a, 01031
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
is available in which the albumin binding site has been deleted. Agarose beads coated with these proteins will bind antibodies and serve to immunoprecipitate antigens from solution. There are differences in the affinity of these proteins for antibodies
Lacrimal Experimental Protocol (4/4/94)
/30/10% Percoll gradient and spin at 500 rpm for 15 min. 30. If doing antibody inhibition experiments, remove antibody and wash BMS two times with warm DMEM/gent (gel) or sterile PBS (coated wells). 31. Cells reside at 60/30% interface. Use
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