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文献和实验推荐一本书--Protein Arrays: Methods and Protocols
. 1. Protein Arrays From cDNA Expression Libraries Weiner, Hendrik; Faupel, Thomas; B?ssow, Konrad pp. 001-014 2. Protein Expression Arrays for Proteomics Gilbert, Michele; Edwards, Todd C.; Albala, Joanna S. pp. 015-024 3. Generation of Protein
minutes at room temperature. 12. Wash plates twice with PBS. 13. Add 100ul/well primary antibody diluted in PBS 14. incubate for 2hr at 37c 15. Wash 5 times with PBS. 16. Add 50ul/well secondary antibody:HRP diluted in PBS. 17. Incubate for 1hr
David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix 5ug GST-fusion protein into total volume 50ul binding buffer.
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