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文献和实验and have mRNA sequences characteristic of 7TM GPCRs, but their endogenous ligands await identification. Most of these receptors have been artificially expressed in cell lines linked to a reporter sys- tem to identify when a ligand binds to the receptor
Blocking of unwanted non-specific staining
Source of unwanted staining, besides poor knowledge of the antibody reactivity and malice, is due to: Endogenous enzymes or fluorochromes. Endogenous biotin. Endogenous antibody binding activity (Fc
5,6. Detector antibodies are especially prone to these kinds of interference: on the one hand, the coupling of an enzyme, like HRP or AP, or a fluorophore can have an influence on the paratope of the antibody and influence its binding specificity
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