- ￥6057
- 赛默飞（Thermo Fisher）
- AOR-001-25UL
- 2025年07月14日
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文献和实验and have mRNA sequences characteristic of 7TM GPCRs, but their endogenous ligands await identification. Most of these receptors have been artificially expressed in cell lines linked to a reporter sys- tem to identify when a ligand binds to the receptor
Staining for Surface Receptors
coverslips. Equilibrate in incubator (~ 25'). 4. Add primary antibody at 1:500 to 1:1000 dilution to media for 30'. Example antibodies: M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; 12CA5 mouse anti-HA (Roche )1:1000
-- Total Volume 10mL, 0.3g dry milk (3%), 100ul 10% Triton X-100 (0.1%), 100ul 100mM CaCl2 (1mM), 500ul 1M Tris-Cl pH 7.5 (50mM). Fixative -- approximately 2ml per coverslip - 37% formaldehyde diluted 1:10 with PBS (150mM NaCl, 10mM NaPi, pH 7.4)
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