- ￥9079
- 赛默飞（Thermo Fisher）
- AMR-062-200UL
- 2025年07月06日
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- 库存：
100
- 规格：
200UL
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文献和实验Wholemount staining of embryos
) at room temperature for 15 to 30''. Use nutator (Clay Adams, Fisher cat#14-062) Remove blocking solution and add primary antibody (500 ul), incubated overnight at 4 degC if sensitivity is not a problem. Antibody is added either as a cell culture
-Cl, pH8.0 1.2114 20% (v/v) Glycerol 20g 4% (w/v) SDS 4g 0.2% Bromophenol blue 0.2g 200mM DTT 20ul 5x Running buffer: 1 L 15.1 g Trizma base (= 125m M) 94g Glycine (= 1.25 M) 50ml 10%SDS (= 0.5%)--add last Do not adjust the pH!! 10x Blotting
between unknown concentration of competitive substance and antibody as above. DAY 2 1. Wash plate with 4x 200ul/well PBS/Tween. 2. Add 50ul/well of antibody and incubate for 2hrs. 3. Wash 4x with 200ul/well PBS/Tween. 4. Add 50ul
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