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文献和实验Determination of Beta-Cell Function: Ion Channel Function in Beta Cells
that influence insulin release. With implementation of the patch-clamp technique it has become possible to analyze ion currents in beta cells under various conditions (e.g., in intact cells or independent of cell metabolism, as whole-cell currents or on a single
phosphate buffer, pH 7, you first need to make two solutions: Solution A: 27.2 g KH2PO4 in 1 L water. Solution B: 34.8 g K2HPO4 in 1 L water. Mix 39 ml Solution A and 61 ml Solution B and then add 100 ml of water.
Beta-Gal staining in primary neurons
Beta-Gal staining in primary neurons Fix cells w/ 4 ml fix soln/10cm or 60 cm dish, 37 C, 5 min Fix soln.: for 100 ml (use w/i 1 wk) 5.4 ml 37 % formaldehyde (Final 2%) 800 ul 25% Glutaraldehyde (fin
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