- ￥5930
- 赛默飞（Thermo Fisher）
- 600-401-682
- 2025年06月24日
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- 库存：
100
- 规格：
100 uG
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文献和实验Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
dilution buffer). Sample volumes should be between 200 and 600 uls. Add 1 ug of antibody to each Ab sample (this may vary for individual antibodies). 13. Incubate on a rotating platform at 4 0 C overnight (or for at least 3 hours
to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10
of beta-mercaptoethanol to 0.1%, glusulase to 0.05%, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37o C. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells
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