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- 赛默飞（Thermo Fisher）
- 56-4301-80
- 2025年07月13日
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文献和实验分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
at -80C thaw to RT before use 3 M KCl may need to autoclave to sterilize; too viscous to filter ? BME+K 500ml BME [5mM K] 4.1 ml 3 M KCl (therefore, 24mM additional
Immunohistochemisty-Enzymatic Protocol
''s instructions. For Research Use Only. Materials Primary Antibodies Unlabeled or biotinylated antigen affinity-purified polyclonal antibodies (R&D Systems'' AF or BAF series) or selected unlabeled or biotinylated monoclonal
Mesoplasma florum: Tn5 Transposase
constant 200 ng of DNA (dilute from 1 ug/ul stock) Use serial dilutions of protein starting at 8 ug/ul 2x dilutions 0, 2, 1, 500, 250, 125, 62.5, 31.25, 15.6, 0 into 20ul TEGX + 200 ng/ul transposon DNA Incubate 30
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