- ￥30194
- 赛默飞（Thermo Fisher）
- 500-P123GBT-500UG
- 2025年07月14日
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500 UG
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文献和实验RNA in situ protocol for DNA and RNA probes
for 3-5 min. with 50 ug/ml proteinase K in PBT**. **When using riboprobes, it is necessary to reduce or eliminate the proteinase K treatment. We use 4 ug/ml proteinase K for 3 min. 11) Stop the protease by washing 2x with PBT containing 2 mg/ml
RNA in situ protocol for DNA and RNA probes
, then 3x with EtOH, 1x with methanol, 1x with 50% methanol: 50% postfix, and 1x with postfix. 8) Postfix the embryos for twenty min. 9) Wash the embryos 3x with PBT. 10) Incubate for 3-5 min. with 50 ug/ml proteinase K in PBT**. **When using
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
. PI is used at 2-5 ug/ml and 7-AAD at 5-10 ug/ml final concentrations. A few seconds of incubation is enough in case of PI, whereas for 7-AAD, 10 minutes at RT or upto 30 minutes on ice is the typical duration of incubation. Hoechst 33342
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