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100
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25 µg
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文献和实验plate) into a gelatin-coated T25 flask. Use a final volume of 3 mL of mES cell growth medium with LIF plus 200 µg/mL hygromycin B. Continue to maintain the cells in a humidified, 5% CO2 incubator at 37ºC. Verification of Gene Expression
Cyanine Dye Purification Protocol
) 20 ml ddH2 O, 0.22 mm filtered 15 mM TEAA (this can be substitutied with 0.750 mL HSB and 49.25 mL ddH2 O, 0.22 mm filtered, to produce 50 mL) Sep-Pak cartridges, C18 (Waters #20515); 1 column per cy dye. Can be reused twice
Mesoplasma florum: Tn5 Transposase
Dilute concentrate with 40% glycerol to bring final to 50% Store aliquots at -80 and -20 Dilute to final 100 ng/ul (1.8 pmol/ul) with storage buffer for use Molarity Gel runs at 55 KD, approximately correct for a 450 AA protein
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