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文献和实验分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
] 1 ml 0.2 M glutamine 1 ml Penn-Strep 40 ul 25 mM araC [10 uM final] +KI per 10ml: 10 ml -KI 25 ul 2mg/ml insulin [5ug/ml final] 67 ul 3M KCl [20mM additional, final = 25mM] sterile filter (if insulin stock
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
RNase-free DNaseI (10 U/µl), 5 µl 0.1 M Tris-Cl pH 8.3, 5 µl 0.5 M KCl, 5 µl 15 mM MgCl 2 phenol extract once precipitate with 5 µl NaAcetate and 200 µl 100 % Ethanol incubate at least for 30 min at -80 °C spin, wash
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