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- 赛默飞（Thermo Fisher）
- 48-0291-80
- 2025年07月15日
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
in microfuge. Aliquot about 450ul to a fresh tube. Freeze in lN2 and store -80C. Fluorometric GUS Mix pr assay 125u1 2 x MUG amd 50u1 100% Methanol prewarm at 37C and at t0 add 75ul extract, mix. When all have been done (up to 50) take out 50
Chemical Co. (St. Louis, Mo, USA). BAY 11-7082 (BAY) was from Calbiochem (La Jolla, Calif, USA). RAW264.7 and U937 cells were purchased from ATCC (Rockville, Md, USA). All other chemicals were of reagent grade. Aggregation-inducing antibody against CD29
, and flasks (sterile tissue culture, gelatin-coated; 48-well, 24-well, 12-well, six-well, 10-cm, T25) Prepare gelatin-coated plasticware just before use by coating the bottom of each dish with 0.1% (w/v) gelatin solution (Sigma). Incubate the dishes
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