- ￥1317
- 赛默飞（Thermo Fisher）
- 47-7311-80
- 2025年07月14日
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文献和实验分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
] 1 ml 0.2 M glutamine 1 ml Penn-Strep 40 ul 25 mM araC [10 uM final] +KI per 10ml: 10 ml -KI 25 ul 2mg/ml insulin [5ug/ml final] 67 ul 3M KCl [20mM additional, final = 25mM] sterile filter (if insulin stock
to each well of any working plates that are to be frozen (-80°C) and subsequently used as culture sources. 2.2 Isolate plasmid templates 1. Warm the lysis buffer (Edge Biosystems Kit) to 37°C to dissolve the SDS. This buffer can be stored at room temperature
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
immediately before use by diluting 1:50 into 1 x PBS. Paraformaldehyde Fixative (4%):4 g solid paraformaldehyde2 mM MgC121.25 mM EGTA (0.25 ml of a .5M EGTA stock pH 8.0) in 100 ml PBS, pH 7.2-7.4Heat H20 to 60°C. Add paraformaldehyde. Add NaOH
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