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文献和实验Intracellular Cytokine Staining Protocol
14 Sa2-8 14-0141 not on blood monocytes Granulocytes Ly6G, GR-1 RB6-8C5 14-5931 blood neutrophils, BM granulocytes and myeloid lineage
9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes
Intracellular Cytokine Staining Protocol
14 Sa2-8 14-0141 not on blood monocytes Granulocytes Ly6G, GR-1 RB6-8C5 14-5931 blood neutrophils, BM granulocytes and myeloid lineage
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