- ￥3564
- 赛默飞（Thermo Fisher）
- 460705
- 2025年07月16日
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- 库存：
100
- 规格：
50 UL (1UG/UL)
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文献和实验is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually
Protocol for mRNA amplification--RT-PCR实验过程
tube (supplied). Add 500ul Buffer RPE (which must contain ethanol) and centrifuge 15 sec at >=8000 x g. Discard flow-through but re-use tube. Add another 500ul Buffer RPE to the RNeasy column. Centrifuge for 2 min at maximum speed. Discard
antibodies in 1% NGS/CMF-PBS. Make 4 mls anti-mouse FTIC diluted 1:150 Make 1.5 mls anti-rabbit FITC diluted 1:200 6. Bind secondary antibodies 45 minutes--use 300 ul/well. 7. Wash 3X with PBST, 5 minutes each wash-use ~1 ml/well. 8.
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