- ￥3856
- 赛默飞（Thermo Fisher）
- 46-9007-42
- 2025年07月12日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 cells may lift as a sheet, which will be broken up by forceful pipetting. 45. Filter the cells through a 40-µm cell strainer. 46. Wash the well with 3 mL of PBS, filter the suspension through the same cell strainer
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The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
that are transferred. 35. Wash the non-adherent cells off the dish, filter the cells through a 40-µm cell strainer, and centrifuge the cells at 400g (1500 rpm) for 5 min at 4°C. 36. Resuspend the cells in OP9 medium, seed 5 x 105
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