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- 赛默飞（Thermo Fisher）
- 46-7221-80
- 2025年07月12日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
) (for Protocols 2-4) Recombinant murine IL-7 (Peprotech 217-17) (for Protocols 2-4) Trypsin solution, 0.25% (Invitrogen 15090046) (for Protocols 1, 2) Dilute stock trypsin to 0.25% with PBS. Equipment Biosafety cabinet Cell strainers (40-µm pore size
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Construction and Characterization of Adenovirus Vectors
), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X
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