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- 赛默飞（Thermo Fisher）
- 46-5698-80
- 2025年07月16日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, except resuspend the cells (~8-10 x 105 cells), in 2 mL of freezing medium for OP9 cells per 10-cm dish. Preferably, freeze cells within the first two to three passages. 14. Aliquot 1 mL of cell suspension per cryovial. 15
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
] 1 ml 0.2 M glutamine 1 ml Penn-Strep 40 ul 25 mM araC [10 uM final] +KI per 10ml: 10 ml -KI 25 ul 2mg/ml insulin [5ug/ml final] 67 ul 3M KCl [20mM additional, final = 25mM] sterile filter (if insulin stock
P la n t C e ll 16, 3386-3399. 25. Riera, M., Figueras, M., Lopez, C., Goday, A., and Pages, M . (2004) Protein kinase C K 2 modulates developmental functions of the abscisic acid responsive protein Ra b l 7 from maize. P r o c. N a tl. A c
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