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文献和实验20X SSC Glycerol (enzyme grade) Sodium Acetate (tri-hydrate) Boric Acid Sodium Hydroxide (1M) Glacial Acetic Acid Succinic anhydride, #23969-0 and 1-methyl-2-pyrrolidinone, # 32863-4 (Aldrich Chemical Co., St. Louis, MO) Diethyl
AFLP: not only for fingerprinting, but for positional cloning
as such: MseI-MseI >>> MseI-EcoRI >>> EcoRI-EcoRI. As discussed below we target the MseI-EcoRI and EcoRI-EcoRI fragments for analysis. 2) Specific ds oligonucleotide adapters (~ 25-30 bp) are ligated to the restricted DNA fragments. 3) Oligos homologous
AFLP: not only for fingerprinting, but for positional cloning
as such: MseI-MseI >>> MseI-EcoRI >>> EcoRI-EcoRI. As discussed below we target the MseI-EcoRI and EcoRI-EcoRI fragments for analysis. 2) Specific ds oligonucleotide adapters (~ 25-30 bp) are ligated to the restricted DNA fragments. 3) Oligos homologous
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