- ￥3497
- 赛默飞（Thermo Fisher）
- 45-7021-82
- 2025年07月09日
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
L of 1X PBS, using the VPW. 25. Activate AEC buffer by adding 4.8 µL of H2 O2 to 10 mL of AEC buffer and filter through a 0.45-µm filter. Add 100 µL per well of activated AEC buffer to each well and incubate up to 5-10 min
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Intracellular Cytokine Staining Protocol
(100ng/ml)(22hr) 2hr/22hr - Monensin B122 IL-2 mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized
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