- ￥1464
- 赛默飞（Thermo Fisher）
- 45-0051-80
- 2025年07月13日
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文献和实验50T/100T 1000/1500 L0050 Mouse anti-Human CD16 mAb,Biotin 50T/100T 800/1200 L0051 Mouse anti-Human CD16 mAb,Biotin 100ug/500ug 800
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 cells may lift as a sheet, which will be broken up by forceful pipetting. 45. Filter the cells through a 40-µm cell strainer. 46. Wash the well with 3 mL of PBS, filter the suspension through the same cell strainer
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