- ￥4149
- 赛默飞（Thermo Fisher）
- 44942G
- 2025年07月09日
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
and stimulated with 0, 0.1, 1 and 10 µg/ml anisomycin for 15 minutes (Lanes 1–4). A Western blot was performed with 8 mg of extracts using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa assays were performed directly in the 96-well plates
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
and stimulated with 0, 0.1, 1 and 10 µg/ml anisomycin for 15 minutes (Lanes 1–4). A Western blot was performed with 8 mg of extracts using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa assays were performed directly in the 96-well plates
with 0.4% trypan blue-PBS for 10 min, and the dead cells were stained blue by trypan blue. The numbers of stained and unstained cells were counted using a hemocytometer. 2.6.Western blot analysis Cells were cultured in six-well microplates to phospho-ERK
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