- ￥4287
- 赛默飞（Thermo Fisher）
- 44636G
- 2025年07月14日
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文献和实验Preparation of Carboxypeptidase Y and Its Properties
with a molecular weight of approximately 61000. The absorptivity of the enzyme that is calculated using the A 280nm of a 1%solution is 15. Carboxypeptidase Y is a diisopropyl-phospho-fluoridate (DFP)-sensitive enzyme. Its inactivation by DFP is accompanied
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
is any nucleotide, R is purine (A, G) and Y is pyrimidine (C, U). Avoid targeting introns, since RNAi only works in the cytoplasm and not within the nucleus. Avoid sequences with > 50% G+C content. Avoid stretches of 4 or more nucleotide repeats
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