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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
E.Z.N.A.TM DNA Fast Protocol For FFPE Tissue
microcentrifuge tube and add 20-100 ul of preheated (70°C) Elution Buffer. Allow tubes to sit for 3 min at room temperature. 13. To elute DNA from the column, centrifuge at 10,000 x g for 1 min. Repeat the elution with a second 20-100 ul of Elution Buffer.
E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
实验步骤 1. Measure the volume of sample and adjust the sample volume to 100ul with DEPC-Water and proceed to step 2. 2. Add 350ul QVL Lysis buffer and mix by vortexing at maximum speed for 15 seconds. When process small
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