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文献和实验Carnation (Dianthus caryophylus L.)
and 50 �M acetosyringone (AS). After cocultivation, the explants were cut into eight radial segments and subcultured onto selection medium until transformed shoots regenerated from the explants.
Reverse Transcription of RNA- 20l rxn
is just as good they are equivalent in units but the companies count them differently. Superscript can be used at higher temperature if secodary structure is a problem. RT-PCR I have put between 0.01 - 5.0l of this rxn into a standard 50 l PCR rxn.
Hemp (Cannabis sativa L.) suspension culture cells were transformed with Agrobacterium tumefaciens strain EHA101 carrying the binary plasmid pNOV3635. The plasmid contains a phosphomannose isomerase (PMI) selectable marker gene. Cells
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