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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
T cells in DMEM for HSC at a density of 2.5 x 106 cells per 100-mm tissue-culture dish in a final volume of 10 mL/dish. 2. On the day of transfection, use the CalPhos Mammalian Transfection Kit to cotransfect 8.6 µg of the HIV packaging
Overview of Formation of G‐Quadruplex Structures
Figure 17.2.1 (A ) Formation of a G‐tetrad. (B ) Anti (left) and syn (right) guanine conformations. (C ) Orientations of G‐strands. (i) All strands in the same direction. (ii) Three strands in one direction, and the fourth in the opposite direction
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
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