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文献和实验Polyclonal and Monoclonal Antibodies
subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed with fusion 4 days later. OR III - Boost 3 days in a row with 15 ug Ag in PBS and proceed with fusion on 4th day. Cell Lines: 1) Myeloma; P3X63-Ag8.653. Origin: BALB/c, non
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
In Situ Hybridization Using Digoxigenin Labeled Probes
down some 100% ethanol in a staining dish to -60 to -80°C 15) Remove the slides individually from the dry ice/ethanol bath and quickly flip off the coverslip with a razor blade and place the slide immediately into the chilled ethanol 16) Allow the ethanol
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