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- 赛默飞（Thermo Fisher）
- 364-5698-80
- 2025年07月16日
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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
] 1 ml 0.2 M glutamine 1 ml Penn-Strep 40 ul 25 mM araC [10 uM final] +KI per 10ml: 10 ml -KI 25 ul 2mg/ml insulin [5ug/ml final] 67 ul 3M KCl [20mM additional, final = 25mM] sterile filter (if insulin stock
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
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