- ￥2029
- 赛默飞（Thermo Fisher）
- 363-7177-80
- 2025年07月16日
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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
Intracellular Immunofluorescent Staining for Flow Cytometry
mouse PEC mIFNγ (100ng/ml) (2hr)/LPS (100ng/ml) (22hr) 2hr/22hr - Brefeldin A C17.8 IL-17A NEW! CD4+CD25- T cells from mouse spleen plus day 10 bone marrow-derived
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