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100 UG
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文献和实验800/1200 L0007 Mouse anti-Human CD3 mAb, Biotin 100ug/500ug 800/2500 L0008 Mouse anti-Human CD4 mAb,FITC 50T/100T 800/1200 L
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.
SANDWICH ELISA FOR DETECTING SECRETE
buffer (Zymed; #00-2011) (a) ABTS chromogen solution (b) Hydrogen Peroxidase solution (c) Citrate buffer 6. Anti-ICAM-1 mAb R6.5 or CL203. Use as 10 ug/ml in PBS to coat plates. 7. Biotinylated mAb: Biotin RR1/1, R6.1 and Biotin R6.5 supplied
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