- ￥4149
- 赛默飞（Thermo Fisher）
- 320700
- 2025年07月11日
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文献和实验Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
ELISPOT (Enzyme-linked ImmunoSPOT)
assay, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible by bulk assay methods are now possible. Limits of detection are below 1/100,000 rendering the assay uniquely useful for monitoring antigen
Intracellular Immunofluorescent Staining for Flow Cytometry
-13A5 IL-10 PBMC Th2 polarizing cultures 6d PMA (50ng/ml) + ionomycin (1ug/ml) Monensin JES3-9D7 IL-12/ IL-23 (p40) PBMC hIFNγ (100ng/ml
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