- ￥3358
- 赛默飞（Thermo Fisher）
- 25-5981-82
- 2025年07月15日
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文献和实验Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
+PTs19I8FlO4qS+YuYoovMwKGJ4/sLfPSHQ+OHSjz4/M+dfumMmlOpqU3ratH+E7e+mI5Yiw7VYztL8dPz6KiLOibFF4uu7eV3pR/5p6dZW6A7u7du8ocPvU5qGFWAPCeLCqaMivXpLt1X3ZXidV/cvp2f6yE+jvDnW3i+sMT2kg/U7+x0MpOBoMDvQHxMypN1Hl/FiC635/6zZzOd3Na+GJvpQc67Ev6c2bPq/ScN9epcQFcoef0ad
ELISPOT (Enzyme-linked ImmunoSPOT)
assay, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible by bulk assay methods are now possible. Limits of detection are below 1/100,000 rendering the assay uniquely useful for monitoring antigen
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