- ￥1913
- 赛默飞（Thermo Fisher）
- 16-7211-82
- 2025年07月16日
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
. 3. Add 100 µL of coating buffer (dual, IL-2, and IFN- ) to the appropriate wells. 4. Seal plate in plastic wrap and incubate overnight at 4°C. Thawing Cells (30 min) 5. Remove frozen PBMCs from the liquid
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
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