- ￥6499
- 赛默飞（Thermo Fisher）
- 16-5883-85
- 2025年06月24日
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
morphology. After the pretreatment the slides should be hybridized within 24 hours. 1) Place the slides in 2X SSC at 65°C for 30 minutes 2) Wash for 2 minutes in 2X SSC at room temperature. 3) Acetylate the slides as follows: Prepare 500 ml of 0.1 M
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
Intracellular Cytokine Staining Protocol
(100ng/ml)(22hr) 2hr/22hr - Monensin B122 IL-2 mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized
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