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文献和实验Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
with anti c-kit MAb and the lineage-negative cells from human bone marrow are incubated with anti-CD34 and anti-CD38 MAb. With a starting population of 2–4 � 10e8 unfractionated mouse bone-marrow cells, a typical isolation procedure yields approx 2 � 10e4 FR
50T/100T 800/1200 L0088 Mouse anti-Human CD34 mAb,Biotin 100ug/500ug 800/2500 L0089 Mouse anti-Human CD35 mAb,FITC 50T/100T 800/1200
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
, harvest the vectors, filter through a 0.45-µm membrane, and store at �80°C. Vectors can be stored for 2-3 mo. Immunoselection of Human CD34+ Cells 5. Dilute CB 1:1 with PBS. Gently layer 35 mL of the diluted blood on 15
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