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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
(I prefer overnight but if you're in a hurry, 3 hours should give a nice probe) 5) Add 20 ug carrier DNA and 1/10 volume 3M NaOAc. Precipitate with 2.5 volumes EtOH for 1 hr @ -70oC or overnight @ -20oC 6) Spin DNA in microfuge 15 min. Remove EtOH
Intracellular Cytokine Staining Protocol
33D1 antigen 33D1 14-5884 splenic DC Monocytes/Macrophages CD11b M1/70 14-0112 also on some T and NK cells
Intracellular Cytokine Staining Protocol
n/a dim 33D1 antigen 33D1 14-5884 splenic DC Monocytes/Macrophages CD11b M1/70 14-0112 also on some T
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