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- 赛默飞（Thermo Fisher）
- 12-5796-82
- 2025年07月16日
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文献和实验Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
well: 5 X 105 live cells 0.5 ml 35 mm: 2.5 X 106 1.5 ml 60mm: 5 X 106 3 ml 100mm: 1.5 X 107 9 ml note, 24w: shake plates vigorously left-right, top-bottom, before placing into incubator, to evenly distribute cells donÕt need to change media after seed
RLCS (Restriction Landmark cDNA Scanning) Protocol
and transfer supernatant containing mRNA to a new RNase-free tube. 15 Repeat 11 - 14 until there is no longer lysate. Extracted mRNA concentration is 100 - 120 ug/mL. Solution volume is 100 ul. A total of 10-12 ug mRNA may be obtained
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