- ￥5451
- 赛默飞（Thermo Fisher）
- 12-0882-82
- 2025年07月16日
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
(I prefer overnight but if you're in a hurry, 3 hours should give a nice probe) 5) Add 20 ug carrier DNA and 1/10 volume 3M NaOAc. Precipitate with 2.5 volumes EtOH for 1 hr @ -70oC or overnight @ -20oC 6) Spin DNA in microfuge 15 min. Remove EtOH
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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