- ￥2912
- 赛默飞（Thermo Fisher）
- 11-0455-85
- 2025年07月14日
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
down some 100% ethanol in a staining dish to -60 to -80°C 15) Remove the slides individually from the dry ice/ethanol bath and quickly flip off the coverslip with a razor blade and place the slide immediately into the chilled ethanol 16) Allow the ethanol
Nick Translation of DNA for CGH
(Gibco; final is 200uM, 10X) 125 ul 1M Tris, pH 7.2 (final 500uM, 10X) 12.5 ul 1M MgCl2 (final 200uM, 10X) 1.7 ul 14.7 M mercaptoethanol (100mM, 10X) 0.5 ul 50 mg/ml BSA (Gibco; final 100 ug/ml, 10X) 95.3 ul dH20
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
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