- ￥7728
- 赛默飞（Thermo Fisher）
- ABS0372102
- 2025年07月13日
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14694
- 规格：
400 µL
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文献和实验2x5 min with Blot buffer. Incubate at RT 1-4 h with secondary antibody in Blot buffer/5%milk. (Use Pierce HRP-coupled secondary Abs at 1:20,000) Wash at least 3x10 min with blot buffer. Drip off, but don''t dry out. Incubate
Use of Escherichia coli Mutator Cells to Mature Antibodies
Despite the power of antibody (Ab) phage-display technology, a problem which can be commonly encountered is the recovery of Abs of low affinity for the antigen (Ag) of interest. Two general strategies can be applied to increase affinity
Construction of Large Nave Fab Libraries
In recent years, a number of single-pot antibody (Ab) libraries have been described, which permit the rapid isolation of high-affinity Abs against large panels of antigens (Ags). Na�ve libraries have been generated by tapping the natural
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