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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
1 cells are used to generate T-lineage cells, and OP9 cells are used to generate B-lineage and myelo-erythroid cells. OP9 medium Phosphate-buffered saline (PBS; Hyclone SH30256) Recombinant human Flt-3/Flk-2 ligand (R&D Systems 308-FK
Size‐Exclusion Chromatography with On‐Line Light Scattering
. Arakawa, T., Yphantis, D.A., Lary, J.W., Narhi, L.O., Lu, H.S., Prestrelski, S.J., Clogston, C.L., Zsebo, K.M., Mendiaz, E.A., Wypych, J., and Langley, K.E. 1991. Glycosylated and unglycosylated recombinant
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