- ￥12315
- 赛默飞（Thermo Fisher）
- A500-042ACF
- 2025年07月12日
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文献和实验Metabolic labeling Immunoprecipitation
) iii)IP 1.Prepare 1.7ml Eppendorf tubes (make sure that cap closes tightly) 2.Add 100-400µl of 35S-labeled culture sup or cell lysate 3.Add mAb (1µg for purified IgG,1µl for ascites,or 50µl for hybridoma supernatant) 4.Add 20µl of ProteinG-agarose
Metabolic labeling Immunoprecipitation
) supplemented w/ 10% dialyzed FCS, & Gln • Chase Medium: Labeling medium (above) + 500µg/ml Cysteine-HCl, 100µg/ml methionine, sterile filtered • Tris-buffered saline (TBS): 20mM Tris, 150mM NaCl, pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma
The production of monoclonal antibody by hybridoma
{Cell Mab (BD), 10% FBS (or HS) ; 5% Origen HCF (hybridoma cloning factor) containing 4mM L-glutamine and antibiotics} to be used for plating hybridomas after the fusion. Hybridoma medium CM - HT (NO AMINOPTERIN) {Cell Mab
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