- ￥2107
- 赛默飞（Thermo Fisher）
- A304810AT
- 2025年07月05日
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11066
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10 UL
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文献和实验Protein A Purification of Antibody
Tris, pH 9-11(8) PBS2. Procedure(1) Dilute Ab 1:5 with Binding Buffer. Filter through a 0.22 m filter.(2) Equilibrate column with at least 2 bed volumes of Binding Buffer(3) Apply Sample.Wash column with 5-6 bed volumes of Binding Buffer. Collect 1 ml
Antibody Staining of Imaginal Discs
in 1 ml 0.1% BSA in tri-PBS, for 5, 10, 15, 20, and 25 minutes, respectively. 10. Replace last wash with 445 ul Tri-PBS, 5 ul 10% BSA, and 40 ul NGS, rock for 30 minutes. 11. Add 10 ul HRP conjugate secondary antibody? and rock for 90 minutes
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
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