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文献和实验wuyf 我想看转录因子sp1对我的目的基因的表达是不是有影响,我用sp1siRNA转染细胞,发现与对照细胞相比,细胞内总RNA量减少。sp1siRNA转染细胞大家有做过的吗? freecell 同样结果重复了几次么?做了几个剂量?每个剂量做了几个重复? ffyy8888 :D因为转染了一些东西进去,或多或少会对细胞状态有影响的,你只要将对照做全,就可以呀,怕啥,多做几组对照
An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1
of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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