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文献和实验Isolation and Propagation of Mouse Liver-Derived Dendritic Cells
, these lymphoid-derived DC can be generated in the absence of granulocyte macrophage colony stimulating factor (GM-CSF), and are CD8+ and Fas ligand+ (CD95L+ ). In the presence of GM-CSF, DC develop directly from myeloid committed precursors, which also give rise
Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
from the inside-out, we also describe a one-hit LV transduction method for constitutive expression of GM-CSF and IL-4 genes, which allows self-differentiation of mouse and human hematopoietic precursor cells into highly viable and potent DCs.
CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
The generation of dendritic cells (DCs) from monocytes and early progenitors in GM-CSF cultures has been the gold standard for in vitro generation of DCs for three decades. However, the most recent evidence suggests that these cultures
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