- ￥1708
- 赛默飞（Thermo Fisher）
- A10113B
- 2025年07月15日
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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
environment with little denaturation. Purified antibodies: Once purified, if the antibody is at less than 0.1 mg/ml add a small amount of detergent or protein (0.1% TWEEN-20 or 1% bovine serum albumin) and some protease inhibitors to the solution
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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